In addition, the NanoDrop 1000 Spectrophotometer has the adequacy to admeasurement awful concentrated samples without dilution

Instrument Description

The Thermo Scientific NanoDrop™ 1000 Spectrophotometer

measures 1 ul samples with top accurateness and reproducibility. The abounding

spectrum (220nm-750nm) spectrophotometer utilizes a patented

sample assimilation technology that employs apparent astriction abandoned to

hold the sample in place. This eliminates the charge for bulky

cuvettes and added sample ascendancy accessories and allows for apple-pie

up in seconds. In addition, the NanoDrop 1000 Spectrophotometer

has the adequacy to admeasurement awful concentrated samples without

dilution (50X college absorption than the samples abstinent by a

standard cuvette spectrophotometer).

Operation

A 1 ul sample is pipetted assimilate the end of a cilia optic cable (the

receiving fiber). A additional cilia optic cable (the antecedent fiber) is then

brought into acquaintance with the aqueous sample causing the aqueous to arch

the gap amid the cilia optic ends. The gap is controlled to both

1mm and 0.2 mm paths. A pulsed xenon beam lamp provides the ablaze

source and a spectrometer utilizing a beeline CCD arrangement is acclimated to

analyze the ablaze afterwards casual through the sample. The apparatus is

controlled by PC based software, and the abstracts is logged in an annal

file on the PC.

Applications

UV/VIS spectrophotometry is simple for samples as baby as 1 ul

using the NanoDrop 1000 Spectrophotometer. The baby sample

requirement and affluence of use accomplish the NanoDrop 1000

Spectrophotometer alluringly ill-fitted for measuring:

• Nucleic acerbic absorption and abstention of nucleic acerbic samples up

to 3700 ng/ul (dsDNA) after concoction

• Beaming dye labeling body of nucleic acerbic microarray

samples

• Purified protein assay (A280) up to 100 mg/ml (BSA)

• Expanded spectrum altitude and quantitation of beaming

dye labeled proteins, conjugates, and metalloproteins

• Bradford Assay assay of protein

• BCA Assay assay of protein

• Lowry Assay assay of protein

• Pierce Protein 660 nm Protein Assay

• Cell body measurements

• General UV-Vis spectrophotometry

Patents

The sample assimilation technology acclimated in the NanoDrop 1000

Spectrophotometer is covered beneath US patents 6,628,382 and

6,809,826. Added patents are pending.