Step to Step Using a Colorimeter

You should firstly affix the colorimeter to the interface and alpha the data-collection software which will analyze it. One of the four blooming amicableness indicator lights will be angry on if it is powered about 5 minutes.

Secondly you should calibrate the colorimeter. Press the button to baddest the actual amicableness ambience for your agreement (430 nm, 470 nm, 565 nm, or 635 nm). Accessible the lid. Insert a cuvette, usually abounding with distilled water, for your bare cuvette (100% transmittance or 0absorbance). Line up one of the bright abandon of the cuvette with the arrow at the top of the cuvette slot. Close the lid. Press the CAL button to activate the arrangement process. Release the CAL button if the red LED begins to flash. The absorbance should now be 0.000 or 0.001. If the LED stops flashing, the arrangement is complete and your assemblage is accessible to aggregate data. Unlike earlier versions of the colorimeter, with this archetypal you do not charge to go to a appropriate arrangement card in our data-collection programs.

Thirdly you should aggregate the data. There are two accepted modes for colorimeter abstracts collection:

Absorbance vs. absorption (Beer’s law) – If you wish to aggregate abstracts in contest with access mode, you can accessible a altered logger pro colorimeter book (with a computer). With calculators or handhelds, you will charge to change from time blueprint to contest with access mode.

Absorbance vs. time – If the Colorimeter is automatically articular by logger pro, it will already be set up to aggregate in this mode. With calculators or handhelds, auto-ID will set the approach to time graph.

Finally you should abode the cuvette with a sample into the colorimeter cuvette slot. Line up one of the bright abandon of the cuvette with the arrow at the top of the cuvette slot. Activate accession abstracts (choose Aggregate or Alpha in the program).In absorbance vs. absorption experiments, you will be prompted to accumulate the absorbance amount (when it stabilizes), and access the absorption of the colorimeter.

You may refer to our website for more info about CFL and LEDtest instruments.